Establishment of a simplified in vitro porcine blood-brain barrier model with high transendothelial electrical resistance.

ABSTRACT: Good in vitro blood-brain barrier (BBB) models that mimic the in vivo BBB phenotype are essential for studies on BBB functionality and for initial screening in drug discovery programmes, as many potential therapeutic drug candidates have poor BBB permeation. Difficulties associated with the availability of human brain tissue, coupled with the time and cost associated with using animals for this kind of research have led to the development of non-human cell culture models. However, most BBB models display a low transendothelial electrical resistance (TEER), which is a measure of the tightness of the BBB. To address these issues we have established and optimised a robust, simple to use in vitro BBB model using porcine brain endothelial cells (PBECs). The PBEC model gives high TEER without th

SUBMITTER: Patabendige A 

PROVIDER: S-EPMC3694297 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature