Analysis of chromatin attachment and partitioning functions of bovine papillomavirus type 1 E2 protein.

ABSTRACT: Recent studies have suggested that the tethering of viral genomes to host cell chromosomes could provide one of the ways to achieve their nuclear retention and partitioning during extrachromosomal maintenance in dividing cells. The data we present here provide firm evidence that the partitioning of the bovine papillomavirus type 1 (BPV1) genome is dependent on the chromatin attachment process mediated by viral E2 protein and its multiple binding sites. On the other hand, the attachment of E2 and the E2-mediated tethering of reporter plasmids to host chromosomes are not necessarily sufficient for efficient partitioning, suggesting that additional E2-dependent activities might be involved in the latter process. The activity of E2 protein in chromatin attachment and partitioning is more sensi

SUBMITTER: Abroi A 

PROVIDER: S-EPMC369436 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature