Genome-wide identification and predictive modeling of tissue-specific alternative polyadenylation.

ABSTRACT:

Motivation

Pre-mRNA cleavage and polyadenylation are essential steps for 3'-end maturation and subsequent stability and degradation of mRNAs. This process is highly controlled by cis-regulatory elements surrounding the cleavage/polyadenylation sites (polyA sites), which are frequently constrained by sequence content and position. More than 50% of human transcripts have multiple functional polyA sites, and the specific use of alternative polyA sites (APA) results in isoforms with variable 3'-untranslated regions, thus potentially affecting gene regulation. Elucidating the regulatory mechanisms underlying differential polyA preferences in multiple cell types has been hindered both by the lack of suitable data on the precise location of cleavage sites, as well as of appropriate tests

SUBMITTER: Hafez D 

PROVIDER: S-EPMC3694680 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature