Ratiometric activatable cell-penetrating peptides provide rapid in vivo readout of thrombin activation.
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ABSTRACT:
SUBMITTER: Whitney M
PROVIDER: S-EPMC3694763 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
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Ratiometric activatable cell-penetrating peptides provide rapid in vivo readout of thrombin activation.
Whitney Michael M Savariar Elamprakash N EN Friedman Beth B Levin Rachel A RA Crisp Jessica L JL Glasgow Heather L HL Lefkowitz Roy R Adams Stephen R SR Steinbach Paul P Nashi Nadia N Nguyen Quyen T QT Tsien Roger Y RY
Angewandte Chemie (International ed. in English) 20121018 1
In real time: thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual-emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage-dependent spatial localization. The most advanced RACPP uses norleucine (Nle)-TPRSFL as a linker that increases sensitivity to thrombin by about 90-fold. ...[more]
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