The influence of 13-cis retinoic acid on human meibomian gland epithelial cells.

ABSTRACT:

Purpose

Meibomian gland dysfunction (MGD) is a primary cause of dry eye disease. One of the risk factors for MGD is exposure to 13-cis retinoic acid (13-cis RA), a metabolite of vitamin A. However, the mechanism is not well understood. We hypothesize that 13-cis RA inhibits cell proliferation, promotes cell death, alters gene and protein expressions, and attenuates cell survival pathways in human meibomian gland epithelial cells.

Methods

To test our hypotheses, immortalized human meibomian gland epithelial cells were cultured with or without 13-cis RA for varying doses and time. Cell proliferation, cell death, gene expression, and proteins involved in proliferation/survival and inflammation were evaluated.

Results

We found that 13-cis RA inhibited cell proliferation,

SUBMITTER: Ding J 

PROVIDER: S-EPMC3694789 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature