Dual-color superresolution microscopy reveals nanoscale organization of mechanosensory podosomes.

ABSTRACT: Podosomes are multimolecular mechanosensory assemblies that coordinate mesenchymal migration of tissue-resident dendritic cells. They have a protrusive actin core and an adhesive ring of integrins and adaptor proteins, such as talin and vinculin. We recently demonstrated that core actin oscillations correlate with intensity fluctuations of vinculin but not talin, suggesting different molecular rearrangements for these components. Detailed information on the mutual localization of core and ring components at the nanoscale is lacking. By dual-color direct stochastic optical reconstruction microscopy, we for the first time determined the nanoscale organization of individual podosomes and their spatial arrangement within large clusters formed at the cell-substrate interface. Superresolution im

SUBMITTER: van den Dries K 

PROVIDER: S-EPMC3694795 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature