NTPDase1 (CD39) controls nucleotide-dependent vasoconstriction in mouse.

ABSTRACT:

Aims

Extracellular nucleotides are vasoactive molecules. The concentrations of these molecules are regulated by ectonucleotidases. In this study, we investigated the role of the blood vessel ectonucleotidase NTPDase1, in the vasoconstrictor effect of nucleotides using Entpd1(-/-) mice.

Methods and results

Immunofluorescence, enzyme histochemistry, and HPLC analysis were used to evaluate both NTPDase expression and activity in arteries and isolated vascular smooth muscle cells (VSMCs). Vascular reactivity was evaluated in vitro and mean arterial blood pressure was recorded in anesthetized mice after nucleotide i.v. infusion. Expression of nucleotide receptors in VSMCs was determined by RT-PCR. Entpd1(-/-) mice displayed a dramatic deficit of nucleotidase activity in blood ves

SUBMITTER: Kauffenstein G 

PROVIDER: S-EPMC3694873 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature