Application of the ITS2 Region for Barcoding Medicinal Plants of Selaginellaceae in Pteridophyta.

ABSTRACT:

Background

Selaginellaceae is a family of nonseed plants with special evolutionary significance. Plants of the family Selaginellaceae are similarly shaped and easily confused, complicating identification via traditional methods. This study explored, for the first time, the use of the DNA barcode ITS2 to identify medicinal plants of the Selaginellaceae family.

Methodology/principal findings

In our study, 103 samples were collected from the main distribution areas in China; these samples represented 34 species and contained almost all of the medicinal plants of Selaginellaceae. The ITS2 region of the genome was amplified from these samples and sequenced using universal primers and reaction conditions. The success rates of the PCR amplification and sequencing were 100%. There w

SUBMITTER: Gu W 

PROVIDER: S-EPMC3694882 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature