Deducing Underlying Mechanisms from Protein Recruitment Data.

ABSTRACT: By using fluorescent labelling techniques, the distribution and dynamics of proteins can be measured within living cells, allowing to study in vivo the response of cells to a triggering event, such as DNA damage. In order to evaluate the reaction rate constants and to identify the proteins and reactions that are essential for the investigated process, mechanistic models are used, which often contain many proteins and associated parameters and are therefore underdetermined by the data. In order to establish criteria for assessing the significance of a model, we present here a systematic investigation of the information that can be reliably deduced from protein recruitment data, assuming that the complete set of reactions that affect the data of the considered protein species is not known. T

SUBMITTER: Lengert L 

PROVIDER: S-EPMC3694963 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature