Serum Antibody Repertoire Profiling Using In Silico Antigen Screen.

ABSTRACT: Serum antibodies are valuable source of information on the health state of an organism. The profiles of serum antibody reactivity can be generated by using a high throughput sequencing of peptide-coding DNA from combinatorial random peptide phage display libraries selected for binding to serum antibodies. Here we demonstrate that the targets of immune response, which are recognized by serum antibodies directed against sequential epitopes, can be identified using the serum antibody repertoire profiles generated by high throughput sequencing. We developed an algorithm to filter the results of the protein database BLAST search for selected peptides to distinguish real antigens recognized by serum antibodies from irrelevant proteins retrieved randomly. When we used this algorithm to analyze se

SUBMITTER: Liu X 

PROVIDER: S-EPMC3695087 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature