Genome conformation capture reveals that the Escherichia coli chromosome is organized by replication and transcription.

ABSTRACT: To fit within the confines of the cell, bacterial chromosomes are highly condensed into a structure called the nucleoid. Despite the high degree of compaction in the nucleoid, the genome remains accessible to essential biological processes, such as replication and transcription. Here, we present the first high-resolution chromosome conformation capture-based molecular analysis of the spatial organization of the Escherichia coli nucleoid during rapid growth in rich medium and following an induced amino acid starvation that promotes the stringent response. Our analyses identify the presence of origin and terminus domains in exponentially growing cells. Moreover, we observe an increased number of interactions within the origin domain and significant clustering of SeqA-binding sequences, sugge

SUBMITTER: Cagliero C 

PROVIDER: S-EPMC3695519 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature