Stability of RNA quadruplex in open reading frame determines proteolysis of human estrogen receptor α.

ABSTRACT: mRNAs encodes not only information that determines amino acid sequences but also additional layers of information that regulate the translational processes. Notably, translational halt at specific position caused by rare codons or stable RNA structures is one of the potential factors regulating the protein expressions and structures. In this study, a quadruplex-forming potential (QFP) sequence derived from an open reading frame of human estrogen receptor α (hERα) mRNA was revealed to form parallel G-quadruplex and halt the translation elongation in vitro. Moreover, when the full-length hERα and variants containing synonymous mutations in the QFP sequence were expressed in cells, translation products cleaved at specific site were observed in quantities dependent on the thermodynamic stabili

SUBMITTER: Endoh T 

PROVIDER: S-EPMC3695533 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature