Lyophilization of semiconducting polymer dot bioconjugates.

ABSTRACT: Semiconducting polymer dot (Pdot) bioconjugates are a new class of ultrabright fluorescent probes. Here, we report a procedure for lyophilizing Pdot bioconjugates so that they successfully retain their optical properties, colloidal stability, and cell-targeting capability during storage. We found that, when Pdot bioconjugates were lyophilized in the presence of 10% sucrose, the rehydrated Pdot bioconjugates did not show any signs of aggregation and exhibited the same hydrodynamic diameters as before lyophilization. The brightness of the lyophilized Pdots was at least as good as before lyophilization, but in some cases, the quantum yield of lyophilized Pdots curiously showed an improvement. Finally, using flow cytometry, we demonstrated that lyophilized Pdot bioconjugates retained their bio

SUBMITTER: Sun W 

PROVIDER: S-EPMC3696440 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature