Proteomic temporal profile of human brain endothelium after oxidative stress.

ABSTRACT:

Background and purpose

because brain endothelial cells exist at the neurovascular interface, they may serve as cellular reporters of brain dysfunction by releasing biomarkers into the circulation.

Methods

we used proteomic techniques to screen conditioned media from human brain endothelial cultures subjected to oxidative stress induced by nitric oxide over 24 hours. Plasma samples from human stroke patients were analyzed by enzyme-linked immunosorbent assay.

Results

in healthy endothelial cells, interaction mapping demonstrated cross-talk involving secreted factors, membrane receptors, and matrix components. In oxidatively challenged endothelial cells, networks of interacting proteins failed to emerge. Instead, inflammatory markers increased, secreted factors oscillat

SUBMITTER: Ning M 

PROVIDER: S-EPMC3696517 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature