Screening of SDS-degrading bacteria from car wash wastewater and study of the alkylsulfatase enzyme activity.

ABSTRACT:

Background and objectives

Sodium dodecyl sulfate (SDS) is one of the main surfactant components in detergents and cosmetics, used in high amounts as a detergent in products such as shampoos, car wash soap and toothpaste. Therefore, its bioremediation by suitable microorganisms is important. Alkylsulfatase is an enzyme that hydrolyses sulfate -ester bonds to give inorganic sulfate and alcohol. The purpose of this study was to isolate SDS-degrading bacteria from Tehran city car wash wastewater, study bacterial alkylsulfatase enzyme activity and identify the alkylsulfatase enzyme coding gene.

Materials and methods

Screening of SDS-degrading bacteria was carried out on basal salt medium containing SDS as the sole source of carbon. Amount of SDS degraded was assayed by methylene

SUBMITTER: Shahbazi R 

PROVIDER: S-EPMC3696852 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature