Isolation and characterization of a thermophilic Bacillus sp. with protease activity isolated from hot spring of Tarabalo, Odisha, India.

ABSTRACT:

Background and objectives

Thermophilic bacteria are less studied but important group of microorganisms due to their ability to produce industrial enzymes.

Materials and methods

In this study, thermophilic bacteria were isolated from hot spring of Tarabalo, India. A bacterium that could tolerate high temperatures was characterized by morphology, biochemistry and sequencing of its 16S rRNA gene. The isolate was screened for protease and amylase activity. Phylogenetic affiliations and G+C content of the isolate was studied.

Results

The bacterium with the ability to tolerate high temperatures was identified as Bacillus sp. both by morphology, biochemistry and sequencing of its 16S rRNA gene. BLAST search analysis of the sequence showed maximum identity with Bacillus amylo

SUBMITTER: Panda MK 

PROVIDER: S-EPMC3696853 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature