Mitochondrial ATP 6 and 8 polymorphisms in irritable bowel syndrome with diarrhea.

ABSTRACT:

Aim

To investigate mitochondrial ATP 6 and 8 polymorphisms in the colon and ileum of patients with irritable bowel syndrome with diarrhea (IBS-D).

Methods

Twenty-eight patients fulfilling the Rome III criteria for IBS-D and 28 healthy subjects were investigated. All study participants underwent screening colonoscopy and mucosal biopsies were obtained from the colon and/or terminal ileum. Genomic DNA was extracted from specimens based on standard protocols. Mitochondrial ATP (MT-ATP) 6 and 8 genes in specimens were polymerase chain reaction amplified and sequenced. Sequencing data were analyzed via Variant Reporter™ Software and compared with the reference sequence from Genbank (accession No. NC_012920) to indicate possible polymorphisms. The protocol was registered at www.cl

SUBMITTER: Wang WF 

PROVIDER: S-EPMC3699044 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature