Relationship between hepatocellular carcinoma and hepatitis B virus genotype with spontaneous YMDD mutations.

ABSTRACT:

Aim

To investigate the relationship between hepatitis B virus (HBV) genotype with spontaneous YMDD mutations and hepatocellular carcinoma (HCC) in HBV-related cirrhosis.

Methods

We investigated 264 liver cirrhosis patients who were not treated with antiviral drugs, including 81 patients with HCC. YMDD mutations were detected by fluorescent hybridization bioprobe polymerase chain reaction (PCR) and melting curve assay using the Diagnosis Kit for HBV YMDD Mutation. Serum HBV genotypes were detected by real-time PCR using genotype-specific TaqMan probes. Statistical analysis was performed according to data type using the t test, χ(2) test and unconditional logistic regression analysis.

Results

In the HCC group, genotype C strains, spontaneous YMDD mutations, and genotype

SUBMITTER: Yang JH 

PROVIDER: S-EPMC3699046 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature