Lineage structure of the human antibody repertoire in response to influenza vaccination.

ABSTRACT: The human antibody repertoire is one of the most important defenses against infectious disease, and the development of vaccines has enabled the conferral of targeted protection to specific pathogens. However, there are many challenges to measuring and analyzing the immunoglobulin sequence repertoire, including that each B cell's genome encodes a distinct antibody sequence, that the antibody repertoire changes over time, and the high similarity between antibody sequences. We have addressed these challenges by using high-throughput long read sequencing to perform immunogenomic characterization of expressed human antibody repertoires in the context of influenza vaccination. Informatic analysis of 5 million antibody heavy chain sequences from healthy individuals allowed us to perform global ch

SUBMITTER: Jiang N 

PROVIDER: S-EPMC3699344 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature