High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells.

ABSTRACT:

Background

The demand of monospecific high affinity binding reagents, particularly monoclonal antibodies, has been steadily increasing over the last years. Enhanced throughput of antibody generation has been addressed by optimizing in vitro selection using phage display which moved the major bottleneck to the production and purification of recombinant antibodies in an end-user friendly format. Single chain (sc)Fv antibody fragments require additional tags for detection and are not as suitable as immunoglobulins (Ig)G in many immunoassays. In contrast, the bivalent scFv-Fc antibody format shares many properties with IgG and has a very high application compatibility.

Results

In this study transient expression of scFv-Fc antibodies in human embryonic kidney (HEK) 293 cells was

SUBMITTER: Jager V 

PROVIDER: S-EPMC3699382 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature