DJ-1-dependent regulation of oxidative stress in the retinal pigment epithelium (RPE).

ABSTRACT:

Background

DJ-1 is found in many tissues, including the brain, where it has been extensively studied due to its association with Parkinson's disease. DJ-1 functions as a redox-sensitive molecular chaperone and transcription regulator that robustly protects cells from oxidative stress.

Methodology

Retinal pigment epithelial (RPE) cultures were treated with H2O2 for various times followed by biochemical and immunohistological analysis. Cells were transfected with adenoviruses carrying the full-length human DJ-1 cDNA and a mutant construct, which has the cysteine residues at amino acid 46, 53 and 106 mutated to serine (C to S) prior to stress experiments. DJ-1 localization, levels of expression and reactive oxygen species (ROS) generation were also analyzed in cells expressing

SUBMITTER: Shadrach KG 

PROVIDER: S-EPMC3699467 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature