Real-time fluorescence loop mediated isothermal amplification for the detection of Acinetobacter baumannii.

ABSTRACT:

Background

Detection of Acinetobacter baumannii has been relying primarily on bacterial culture that often fails to return useful results in time. Although DNA-based assays are more sensitive than bacterial culture in detecting the pathogen, the molecular results are often inconsistent and challenged by doubts on false positives, such as those due to system- and environment-derived contaminations. In addition, these molecular tools require expensive laboratory instruments. Therefore, establishing molecular tools for field use require simpler molecular platforms. The loop-mediated isothermal amplification method is relatively simple and can be improved for better use in a routine clinical bacteriology laboratory. A simple and portable device capable of performing both the amplificat

SUBMITTER: Wang Q 

PROVIDER: S-EPMC3699609 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature