Hydrophobic derivatization of N-linked glycans for increased ion abundance in electrospray ionization mass spectrometry.

ABSTRACT: A library of neutral, hydrophobic reagents was synthesized for use as derivatizing agents in order to increase the ion abundance of N-linked glycans in electrospray ionization mass spectrometry (ESI MS). The glycans are derivatized via hydrazone formation and are shown to increase the ion abundance of a glycan standard more than 4-fold. Additionally, the data show that the systematic addition of hydrophobic surface area to the reagent increases the glycan ion abundance, a property that can be further exploited in the analysis of glycans. The results of this study will direct the future synthesis of hydrophobic reagents for glycan analysis using the correlation between hydrophobicity and theoretical non-polar surface area calculation to facilitate the development of an optimum tag for glyca

SUBMITTER: Walker SH 

PROVIDER: S-EPMC3700344 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature