Cellulase activity mapping of Trichoderma reesei cultivated in sugar mixtures under fed-batch conditions.

ABSTRACT:

Background

On-site cellulase production using locally available lignocellulosic biomass (LCB) is essential for cost-effective production of 2nd-generation biofuels. Cellulolytic enzymes (cellulases and hemicellulases) must be produced in fed-batch mode in order to obtain high productivity and yield. To date, the impact of the sugar composition of LCB hydrolysates on cellulolytic enzyme secretion has not been thoroughly investigated in industrial conditions.

Results

The effect of sugar mixtures (glucose, xylose, inducer) on the secretion of cellulolytic enzymes by a glucose-derepressed and cellulase-hyperproducing mutant strain of Trichoderma reesei (strain CL847) was studied using a small-scale protocol representative of the industrial conditions. Since production of cellulo

SUBMITTER: Jourdier E 

PROVIDER: S-EPMC3700819 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature