Different selective effects on rhizosphere bacteria exerted by genetically modified versus conventional potato lines.

ABSTRACT:

Background

In this study, we assessed the actively metabolizing bacteria in the rhizosphere of potato using two potato cultivars, i.e. the genetically-modified (GM) cultivar Modena (having tubers with altered starch content) and the near-isogenic non-GM cultivar Karnico. To achieve our aims, we pulse-labelled plants at EC90 stage with (13)C-CO2 and analysed their rhizosphere microbial communities 24 h, 5 and 12 days following the pulse. In the analyses, phospholipid fatty acid/stable isotope probing (PLFA-SIP) as well as RNA-SIP followed by reverse transcription and PCR-DGGE and clone library analysis, were used to determine the bacterial groups that actively respond to the root-released (13)C labelled carbonaceous compounds.

Methodology/principal findings

The PLFA-SIP data

SUBMITTER: Dias AC 

PROVIDER: S-EPMC3700926 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature