Efficient high-throughput sequencing of a laser microdissected chromosome arm.

ABSTRACT:

Background

Genomic sequence assemblies are key tools for a broad range of gene function and evolutionary studies. The diploid amphibian Xenopus tropicalis plays a pivotal role in these fields due to its combination of experimental flexibility, diploid genome, and early-branching tetrapod taxonomic position, having diverged from the amniote lineage ~360 million years ago. A genome assembly and a genetic linkage map have recently been made available. Unfortunately, large gaps in the linkage map attenuate long-range integrity of the genome assembly.

Results

We laser dissected the short arm of X. tropicalis chromosome 7 for next generation sequencing and computational mapping to the reference genome. This arm is of particular interest as it encodes the sex determination locus, b

SUBMITTER: Seifertova E 

PROVIDER: S-EPMC3701504 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature