Alternative polyadenylation sites reveal distinct chromatin accessibility and histone modification in human cell lines.

ABSTRACT:

Motivation

In addition to alternative splicing, alternative polyadenylation has also been identified as a critical and prevalent regulatory mechanism in human gene expression. However, the mechanism of alternative polyadenylation selection and the involved factors is still largely unknown.

Results

We use the ENCODE data to scan DNA functional elements, including chromatin accessibility and histone modification, around transcript cleavage sites. Our results demonstrate that polyadenylation sites tend to be less sensitive to DNase I. However, these polyadenylation sites have preference in nucleosome-depleted regions, indicating the involvement of chromatin higher-order structure rather than nucleosomes in the resultant lower chromatin accessibility. More interestingly, for gen

SUBMITTER: Lee CY 

PROVIDER: S-EPMC3702257 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature