Role of MUC20 overexpression as a predictor of recurrence and poor outcome in colorectal cancer.

ABSTRACT:

Background

Colorectal cancer (CRC) remains one of the most common cancers worldwide. We observed that MUC20 was significantly up-regulated in CRC patients with poor prognosis based on the microarray analysis. However, little is known about the role of MUC20 in CRC.

Methods

Microarray experiments were performed on the Affymetrix U133 plus 2.0 GeneChip Array. The protein and mRNA levels of MUC20 were examined by immunohistochemistry (IHC) and Real-Time quantitative PCR (RT-qPCR) in CRC tissues and adjacent noncancerous tissues (ANCT). ShRNA and overexpression plasmids were used to regulate MUC20 expression in CRC cell lines in vitro; wound healing, Transwell migration assays, and Western blotting were used to detect migration and invasion changes.

Results

MUC20 was one

SUBMITTER: Xiao X 

PROVIDER: S-EPMC3702436 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature