De novo transcriptome sequencing of axolotl blastema for identification of differentially expressed genes during limb regeneration.

ABSTRACT:

Background

Salamanders are unique among vertebrates in their ability to completely regenerate amputated limbs through the mediation of blastema cells located at the stump ends. This regeneration is nerve-dependent because blastema formation and regeneration does not occur after limb denervation. To obtain the genomic information of blastema tissues, de novo transcriptomes from both blastema tissues and denervated stump ends of Ambystoma mexicanum (axolotls) 14 days post-amputation were sequenced and compared using Solexa DNA sequencing.

Results

The sequencing done for this study produced 40,688,892 reads that were assembled into 307,345 transcribed sequences. The N50 of transcribed sequence length was 562 bases. A similarity search with known proteins identified 39,200 diffe

SUBMITTER: Wu CH 

PROVIDER: S-EPMC3702472 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature