Genomic and phenotypic characterization of in vitro-generated Chlamydia trachomatis recombinants.

ABSTRACT:

Background

Pre-genomic and post-genomic studies demonstrate that chlamydiae actively recombine in vitro and in vivo, although the molecular and cellular biology of this process is not well understood. In this study, we determined the genome sequence of twelve Chlamydia trachomatis recombinants that were generated in vitro under antibiotic selection. These strains were used to explore the process of recombination in Chlamydia spp., including analysis of candidate recombination hotspots, and to correlate known C. trachomatis in vitro phenotypes with parental phenotypes and genotypes.

Results

Each of the 190 examined recombination events was the product of homologous recombination, and no candidate targeting motifs were identified at recombination sites. There was a single dele

SUBMITTER: Jeffrey BM 

PROVIDER: S-EPMC3703283 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature