Laserspray ionization imaging of multiply charged ions using a commercial vacuum MALDI ion source.

ABSTRACT: This is the first report of imaging mass spectrometry (MS) from multiply charged ions at vacuum. Laserspray ionization (LSI) was recently extended to applications at vacuum producing electrospray ionization-like multiply charged ions directly from surfaces using a commercial intermediate pressure matrix-assisted laser desorption/ionization ion mobility spectrometry (IMS) MS instrument. Here, we developed a strategy to image multiply charged peptide ions. This is achieved by the use of 2-nitrophloroglucinol as matrix for spray deposition onto the tissue section and implementation of "soft" acquisition conditions including lower laser power and ion accelerating voltages similar to electrospray ionization-like conditions. Sufficient ion abundance is generated by the vacuum LSI method to emplo

SUBMITTER: Inutan ED 

PROVIDER: S-EPMC3703845 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature