Minimally modified LDL upregulates endothelin type A receptors in rat coronary arterial smooth muscle cells.

ABSTRACT: Minimally modified low-density lipoprotein (mmLDL) is a risk factor for cardiovascular disease. The present study investigated the effects of mmLDL on the expression of endothelin type A (ET(A)) receptors in coronary arteries. Rat coronary arteries were organ-cultured for 24 h. The contractile responses were recorded using a myographic system. ET(A) receptor mRNA and protein expressions were determined using real-time PCR and western blotting, respectively. The results showed that organ-culturing in the presence of mmLDL enhanced the arterial contractility mediated by the ET(A) receptor in a concentration-dependent and time-dependent manner. Culturing with mmLDL (10  μ g/mL) for 24 h shifted the concentration-contractile curves toward the left significantly with increased E(max) of 228% ±

SUBMITTER: Li J 

PROVIDER: S-EPMC3703896 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature