Hydrocortisone stimulates neurite outgrowth from mouse retinal explants by modulating macroglial activity.

ABSTRACT:

Purpose

There is mounting evidence that retinal ganglion cells (RGCs) require a complex milieu of trophic factors to enhance cell survival and axon regeneration after optic nerve injury. The authors' goal was to examine the contribution of components of a combination of hormones, growth factors, steroids, and small molecules to creating a regenerative environment and to determine if any of these components modulated macroglial behavior to aid in regeneration.

Methods

Postnatal day 7 mouse retinal explants embedded in collagen were used as an in vitro model of neurite regeneration. Explants were treated with the culture supplements fetal bovine serum, N2, and G5 and a mixture of G5 and N2 components, designated enhanced N2 (EN2). Explants were evaluated for neurite outgrowth

SUBMITTER: Toops KA 

PROVIDER: S-EPMC3704200 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature