Transcriptional profiling of Drosophila S2 cells in early response to Drosophila C virus.

ABSTRACT:

Background

The innate immune response like phagocytosis, encapsulation and antimicrobial peptide (AMP) production often occur in the early stage of host-pathogen interactions in Drosophila melanogaster. To investigate the Drosophila early immune response to Drosophila C virus, we characterized the DCV infection-response transcriptome of Drosophila Schneider 2 (S2) cells at one hour post inoculation.

Method

The total RNA was extracted from treated S2 cells by using Trizol reagent and then analyzed by CapitalBio Corp for Drosophila GeneChip (Affymetrix) assay. Then the results of signaling pathway and protein interaction about these genes were analyzed by MAS 3.0 software.

Results

Most significantly affected genes (656 genes) by DCV infection were regulated as the same

SUBMITTER: Zhu F 

PROVIDER: S-EPMC3704779 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature