Extensive transcriptional heterogeneity revealed by isoform profiling.

ABSTRACT: Transcript function is determined by sequence elements arranged on an individual RNA molecule. Variation in transcripts can affect messenger RNA stability, localization and translation, or produce truncated proteins that differ in localization or function. Given the existence of overlapping, variable transcript isoforms, determining the functional impact of the transcriptome requires identification of full-length transcripts, rather than just the genomic regions that are transcribed. Here, by jointly determining both transcript ends for millions of RNA molecules, we reveal an extensive layer of isoform diversity previously hidden among overlapping RNA molecules. Variation in transcript boundaries seems to be the rule rather than the exception, even within a single population of yeast cells

SUBMITTER: Pelechano V 

PROVIDER: S-EPMC3705217 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature