Insights from the molecular characterization of mercury stress proteins identified by proteomics in E.coli nissle 1917.

ABSTRACT: Differently expressed proteins in probiotic Escherichia coli nissle 1917 under mercury stress identified by using a proteomic approach. We applied to separate proteins by using two-dimensional gel electrophoresis and proteins were identified using MALDI-TOF-MS using PMF, by mascot database search using the NCBI database. we identified six proteins after exposure to mercury stress with respect to different functional classes. It is useful to understand the molecular insights into mercury stress in probiotic E. coli. Next we describe a structure generated by homology modelling and functional domain identification; it is interesting to study the impact of stress on protein structures. MS characterization and computational methods together provide the opportunity to examine the impact of stres

SUBMITTER: Seshapani P 

PROVIDER: S-EPMC3705621 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature