The extracellular microenvironment explains variations in passive drug transport across different airway epithelial cell types.

ABSTRACT:

Purpose

We sought to identify key variables in cellular architecture and physiology that might explain observed differences in the passive transport properties of small molecule drugs across different airway epithelial cell types.

Methods

Propranolol (PR) was selected as a weakly basic, model compound to compare the transport properties of primary (NHBE) vs. tumor-derived (Calu-3) cells. Differentiated on Transwell™ inserts, the architecture of pure vs. mixed cell co-cultures was studied with confocal microscopy followed by quantitative morphometric analysis. Cellular pharmacokinetic modeling was used to identify parameters that differentially affect PR uptake and transport across these two cell types.

Results

Pure Calu-3 and NHBE cells possessed different structural

SUBMITTER: Min KA 

PROVIDER: S-EPMC3706189 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature