Investigating host dependence of xylose utilization in recombinant Saccharomyces cerevisiae strains using RNA-seq analysis.

ABSTRACT:

Background

Xylose-based ethanol production by recombinant S. cerevisiae is of great interest to basic and applied bioenergy research. By expressing three different fungal pathways in two S. cerevisiae hosts respectively, we found that the xylose utilization efficiency by recombinant S. cerevisiae depends not only on the choice of xylose pathway but also on the choice of host, exhibiting an obvious host or context dependence. To investigate molecular mechanisms of this context dependence, we applied RNA-seq analysis in this study for a systematic characterization of the xylose utilization via different pathways in different S. cerevisiae hosts.

Results

Based on the RNA-seq analysis, the transcripts that were regulated during xylose utilization have been identified. Three tran

SUBMITTER: Feng X 

PROVIDER: S-EPMC3706274 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature