Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules.

ABSTRACT:

Background

The ability to adapt to environments with fluctuating nutrient availability is vital for bacterial survival. Although essential for growth, few nitrogen metabolism genes have been identified or fully characterised in mycobacteria and nitrogen stress survival mechanisms are unknown.

Results

A global transcriptional analysis of the mycobacterial response to nitrogen stress, showed a significant change in the differential expression of 16% of the Mycobacterium smegmatis genome. Gene expression changes were mapped onto the metabolic network using Active Modules for Bipartite Networks (AMBIENT) to identify metabolic pathways showing coordinated transcriptional responses to the stress. AMBIENT revealed several key features of the metabolic response not identified by KEG

SUBMITTER: Williams KJ 

PROVIDER: S-EPMC3706326 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature