Conditionally-live attenuated SIV upregulates global T effector memory cell frequency under replication permissive conditions.

ABSTRACT:

Background

Live attenuated SIV induces potent protection against superinfection with virulent virus; however the mechanism of this vaccine effect is poorly understood. Such knowledge is important for the development of clinically acceptable vaccine modalities against HIV.

Results

Using a novel, doxycycline dependent, replication-competent live-attenuated SIVmac239Δnef (SIV-rtTAΔnef), we show that under replication-permissive conditions SIV-rtTAΔnef is fully viable. Twelve rhesus macaques were infected with a peak plasma vRNA on average two log10 lower than in 6 macaques infected with unconditionally replication-competent SIVΔnef. Consistent with the attenuated phenotype of the viruses the majority of animals displayed low or undetectable levels of viraemia by 42-84 days afte

SUBMITTER: Manoussaka MS 

PROVIDER: S-EPMC3706341 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature