Tumor-associated copy number changes in the circulation of patients with prostate cancer identified through whole-genome sequencing.

ABSTRACT:

Background

Patients with prostate cancer may present with metastatic or recurrent disease despite initial curative treatment. The propensity of metastatic prostate cancer to spread to the bone has limited repeated sampling of tumor deposits. Hence, considerably less is understood about this lethal metastatic disease, as it is not commonly studied. Here we explored whole-genome sequencing of plasma DNA to scan the tumor genomes of these patients non-invasively.

Methods

We wanted to make whole-genome analysis from plasma DNA amenable to clinical routine applications and developed an approach based on a benchtop high-throughput platform, that is, Illuminas MiSeq instrument. We performed whole-genome sequencing from plasma at a shallow sequencing depth to establish a genome-wide

SUBMITTER: Heitzer E 

PROVIDER: S-EPMC3707016 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature