FDRAnalysis: a tool for the integrated analysis of tandem mass spectrometry identification results from multiple search engines.

ABSTRACT: Confident identification of peptides via tandem mass spectrometry underpins modern high-throughput proteomics. This has motivated considerable recent interest in the postprocessing of search engine results to increase confidence and calculate robust statistical measures, for example through the use of decoy databases to calculate false discovery rates (FDR). FDR-based analyses allow for multiple testing and can assign a single confidence value for both sets and individual peptide spectrum matches (PSMs). We recently developed an algorithm for combining the results from multiple search engines, integrating FDRs for sets of PSMs made by different search engine combinations. Here we describe a web-server and a downloadable application that makes this routinely available to the proteomics comm

SUBMITTER: Wedge DC 

PROVIDER: S-EPMC3707089 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature