Integrative omics analysis reveals the importance and scope of translational repression in microRNA-mediated regulation.

ABSTRACT: MicroRNAs (miRNAs) are key post-transcriptional regulators that inhibit gene expression by promoting mRNA decay and/or suppressing translation. However, the relative contributions of these two mechanisms to gene repression remain controversial. Early studies favor a translational repression-centric scenario, whereas recent large-scale studies suggest a dominant role of mRNA decay in miRNA regulation. Here we generated proteomics data for nine colorectal cancer cell lines and integrated them with matched miRNA and mRNA expression data to infer and characterize miRNA-mediated regulation. Consistent with previous reports, we found that 8mer site, site positioning within 3'UTR, local AU-rich context, and additional 3' pairing could all help boost miRNA-mediated mRNA decay. However, these seque

SUBMITTER: Liu Q 

PROVIDER: S-EPMC3708174 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature