Interaction between allergy and innate immunity: model for eosinophil regulation of epithelial cell interferon expression.

ABSTRACT:

Background

Eosinophils in asthmatic airways are associated with risk of exacerbations. The most common cause of asthma exacerbations is viral respiratory infections, particularly human rhinovirus (HRV).

Objective

To determine the mechanism by which eosinophils may influence virus-induced responses.

Methods

We used an in vitro coculture model of primary human eosinophils and the BEAS-2B epithelial cell line either stimulated with HRV1A infection or polyinosinic-polycytidylic acid (poly[I:C]). The messenger RNA (mRNA) expression of interferon (IFN) β1 and IFN-λ1 was assessed by quantitative reverse-transcriptase polymerase chain reaction and the protein level of IFN- λ1 by enzyme-linked immunosorbent assay.

Results

Both poly(I:C) and HRV1A infection induced BEAS-

SUBMITTER: Mathur SK 

PROVIDER: S-EPMC3708694 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature