A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max.

ABSTRACT:

Background

Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.

Findings

Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described.

Conclusions

The VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to st

SUBMITTER: Kandoth PK 

PROVIDER: S-EPMC3708766 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature