Establishment and characterization of a telomerase immortalized human gingival epithelial cell line.

ABSTRACT:

Background and objective

Gingival keratinocytes are used in model systems to investigate the interaction between periodontal bacteria and the epithelium in the initial stages of the periodontal disease process. Primary gingival epithelial cells (GECs) have a finite lifespan in culture before they enter senescence and cease to replicate, while epithelial cells immortalized with viral proteins can exhibit chromosomal rearrangements. The aim of this study was to generate a telomerase immortalized human gingival epithelial cell line and compare its in vitro behaviour to that of human GECs.

Material and methods

Human primary GECs were immortalized with a bmi1/hTERT combination to prevent cell cycle triggers of senescence and telomere shortening. The resultant cell-line, telomeras

SUBMITTER: Moffatt-Jauregui CE 

PROVIDER: S-EPMC3709015 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature