Construction of the coding sequence of the transcription variant 2 of the human Renalase gene and its expression in the prokaryotic system.

ABSTRACT: Renalase is a recently discovered protein, involved in regulation of blood pressure in humans and animals. Although several splice variants of human renalase mRNA transcripts have been recognized, only one protein product, hRenalase1, has been found so far. In this study, we have used polymerase chain reaction (PCR)-based amplification of individual exons of the renalase gene and their joining for construction of full-length hRenalase2 coding sequence followed by expression of hRenalase2 as a polyHis recombinant protein in Escherichia coli cells. To date this is the first report on synthesis and purification of hRenalase2. Applicability of this approach was verified by constructing hRenalase1 coding sequence, its sequencing and expression in E. coli cells. hRenalase1 was used for generatio

SUBMITTER: Fedchenko VI 

PROVIDER: S-EPMC3709811 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature