Shuttle vectors for facile gap repair cloning and integration into a neutral locus in Candida albicans.

ABSTRACT: Candida albicans is the most prevalent fungal pathogen of humans. The current techniques used to construct C. albicans strains require integration of exogenous DNA at ectopic locations, which can exert position effects on gene expression that can confound the interpretation of data from critical experiments such as virulence assays. We have identified a large intergenic region, NEUT5L, which facilitates the integration and expression of ectopic genes. To construct and integrate inserts into this novel locus, we re-engineered yeast/bacterial shuttle vectors by incorporating 550 bp of homology to NEUT5L. These vectors allow rapid, facile cloning through in vivo recombination (gap repair) in Saccharomyces cerevisiae and efficient integration of the construct into the NEUT5L locus. Other usefu

SUBMITTER: Gerami-Nejad M 

PROVIDER: S-EPMC3709822 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature