Identification and characterization of microRNAs from Entamoeba histolytica HM1-IMSS.

ABSTRACT:

Background

Entamoeba histolytica is the causative agent of amebiasis, a disease that is a major source of morbidity and mortality in the developing world. MicroRNAs (miRNAs) are a large group of non-coding RNAs that play important roles in regulating gene expression and protein translation in animals. Genome-wide identification of miRNAs is a critical step to facilitating our understanding of genome organization, genome biology, evolution, and post-transcriptional regulation.

Methodology/principal findings

We sequenced a small RNA library prepared from a culture of trophozoites of Entamoeba histolytica Strain HM1-IMSS using a deep DNA sequencing approach. Deep sequencing yielded 16 million high-quality short sequence reads containing a total of 5 million non-redundant sequen

SUBMITTER: Mar-Aguilar F 

PROVIDER: S-EPMC3709888 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature