Male-biased genes in catfish as revealed by RNA-Seq analysis of the testis transcriptome.

ABSTRACT:

Background

Catfish has a male-heterogametic (XY) sex determination system, but genes involved in gonadogenesis, spermatogenesis, testicular determination, and sex determination are poorly understood. As a first step of understanding the transcriptome of the testis, here, we conducted RNA-Seq analysis using high throughput Illumina sequencing.

Methodology/principal findings

A total of 269.6 million high quality reads were assembled into 193,462 contigs with a N50 length of 806 bp. Of these contigs, 67,923 contigs had hits to a set of 25,307 unigenes, including 167 unique genes that had not been previously identified in catfish. A meta-analysis of expressed genes in the testis and in the gynogen (double haploid female) allowed the identification of 5,450 genes that are prefere

SUBMITTER: Sun F 

PROVIDER: S-EPMC3709890 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature